t(1;19) E2A-PBX1(TCF3-PBX1)Fusion detection Qualitative One step RT-PCR Kit

The t(1;19) is detected in about 5–6% of childhood ALL and in about 3% of adult ALL. This translocation can be found in a balanced (25%) or unbalanced (75%) form -19, +der(19)t(1;19) in which two normal chromosomes 1 are present. In both pediatric and adult patients this translocation occurs almost exclusively in pre-B-ALL expressing cytoplasmic Igµ, even though it has been reported sporadically in pro-B-ALL and common ALL (<1%) as well as in rare cases of T-ALL and AML. Most cases carrying the t(1;19) express a typical immunophenotype with homogeneous expression of CD19, CD10, CD9, complete absence of CD34, and at least partial absence of CD20. Moreover, the t(1;19) correlates with the presence of known clinical high-risk features, such as elevated cell count, high serum lactate dehydrogenase levels and central nervous  system involvement. This kit has been manufactured for investigational use only (IUO) to qualitatively detect the E2A-PBX1 fusion transcripts in ALL cases (Pre B-ALL and rarely Pro B-ALL).