Description
(AmirPayvand Research & Development Company) AP-RAD Dengue/Chikungunya/Zika Virus Multiplex rRT-PCR Kit is designed to detect DENV, CHIKV, and ZIKV simultaneously using one step, Real-Time RT-PCR technique, contributing to public health enhancement by accelerating infectious fever diagnosis and treatment.
Arboviruses are transmitted by arthropods, including those responsible for the current significant global burden, Chikungunya (genus Alphavirus, family Togaviridae), Dengue, and Zika virus (genus Flavivirus, family Flaviviridae). Dengue virus (DENV), Zika virus (ZIKV), and Chikungunya virus (CHIKV) are single-stranded RNA viruses mainly spread to people through infected Aedes aegypti or Aedes albopictus mosquitos’ bites and lead to diseases with similar clinical manifestations like fever, joint and musculoskeletal pain, headache and rashes. Although the mortality rate of these infections is low, they can still pose a considerable threat to life if left untreated by shifting to severe phases. Severe Dengue, also known as Dengue Hemorrhagic Fever (DHF) or Dengue Shock Syndrome (DSS), can result in severe bleeding, organ impairment, and death in some cases. While, severe manifestations of Zika fever can be neurological complications such as Guillain-Barre syndrome in adults and fetus microcephaly in infected mothers. Preterm birth and miscarriage may also occur in pregnant women as a result of the virus. Moreover, patients with Chikungunya fever may develop persistent joint issues like arthritis and further related disabilities. Due to the overlapping symptoms of Zika, Chikungunya, and Dengue fever, healthcare practitioners routinely prescribe molecular or serological tests for accurate diagnosis. The virus detection would be imprecise via serological tests duo to possible cross-reactions, while reverse transcription polymerase chain reaction (RT–PCR) is considered the gold standard assay for viral genome detection.
